DYn-2 Based Identification of Arabidopsis Sulfenomes.
Mol Cell Proteomics
; 14(5): 1183-200, 2015 May.
Article
em En
| MEDLINE
| ID: mdl-25693797
ABSTRACT
Identifying the sulfenylation state of stressed cells is emerging as a strategic approach for the detection of key reactive oxygen species signaling proteins. Here, we optimized an in vivo trapping method for cysteine sulfenic acids in hydrogen peroxide (H2O2) stressed plant cells using a dimedone based DYn-2 probe. We demonstrated that DYn-2 specifically detects sulfenylation events in an H2O2 dose- and time-dependent way. With mass spectrometry, we identified 226 sulfenylated proteins after H2O2 treatment of Arabidopsis cells, residing in the cytoplasm (123); plastid (68); mitochondria (14); nucleus (10); endoplasmic reticulum, Golgi and plasma membrane (7) and peroxisomes (4). Of these, 123 sulfenylated proteins have never been reported before to undergo cysteine oxidative post-translational modifications in plants. All in all, with this DYn-2 approach, we have identified new sulfenylated proteins, and gave a first glance on the locations of the sulfenomes of Arabidopsis thaliana.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ácidos Sulfênicos
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Sondas Moleculares
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Processamento de Proteína Pós-Traducional
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Arabidopsis
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Cicloexanonas
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Proteínas de Arabidopsis
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article