SUZ12 RNA interference inhibits the invasion of gastric carcinoma cells.

ABSTRACT

In order to observe the regulation effects of SUZ12 gene to the invasion of gastric carcinoma cells and the mechanisms, the expressions of SUZ12 protein in 86 patients with different infiltrating degree (Tis-T4), lymph node metastasis (N0-N3) and far metastasis (M0-M1) were detected by immunohistochemical staining. The siRNA to SUZ12 was designed and synthesized and transfected into MKN28 cells. The effects of SUZ12 siRNA to cell invasion were detected by soft agar colony forming test and Transwell cabin model. The related protein was detected by Western-Blot. We found the expression of SUZ12 in gastric carcinoma tissues (23.58±9.89%) was obviously higher than that in para-cancer tissue (1.12%±0.12%) (p<0.01). The expression of SUZ12 was related to the infiltrating degree, and demonstrated an increasing tendency from Tis-T4, or from N0-N3. The expression of SUZ12 in M0 was obviously lower than in M1, p<0.01. The level of SUZ12 was descent obviously after transfected SUZ12 siRNA (p<0.01). The number of cell clone was reduced in dose dependent of siRNA and the cells permeated through filter membrane were decreased after transfected siRNA. Inhibition of SUZ12 caused an obviously descent of VEGF, MMP-2 and MMP-9 (0.22±0.06, 0.12±0.03, 0.08±0.02) compared to non transfected group (0.87±0.08, 0.92±0.16, 1.05±0.18) respectively (p<0.01). We draw the conclusion that the expression of SUZ12 is increasing along with the degree of infiltrating and metastasis of gastric carcinoma. SUZ12 siRNA inhibits the invasion of gastric carcinoma cells and the expression of VEGF, MMP-2 and MMP-9.