Brain structure. Cell types in the mouse cortex and hippocampus revealed by single-cell RNA-seq.
Science
; 347(6226): 1138-42, 2015 Mar 06.
Article
em En
| MEDLINE
| ID: mdl-25700174
ABSTRACT
The mammalian cerebral cortex supports cognitive functions such as sensorimotor integration, memory, and social behaviors. Normal brain function relies on a diverse set of differentiated cell types, including neurons, glia, and vasculature. Here, we have used large-scale single-cell RNA sequencing (RNA-seq) to classify cells in the mouse somatosensory cortex and hippocampal CA1 region. We found 47 molecularly distinct subclasses, comprising all known major cell types in the cortex. We identified numerous marker genes, which allowed alignment with known cell types, morphology, and location. We found a layer I interneuron expressing Pax6 and a distinct postmitotic oligodendrocyte subclass marked by Itpr2. Across the diversity of cortical cell types, transcription factors formed a complex, layered regulatory code, suggesting a mechanism for the maintenance of adult cell type identity.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Córtex Somatossensorial
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Oligodendroglia
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Análise de Sequência de RNA
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Região CA1 Hipocampal
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Análise de Célula Única
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Interneurônios
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article