Targeted nanodiamonds as phenotype-specific photoacoustic contrast agents for breast cancer.
Nanomedicine (Lond)
; 10(4): 573-87, 2015 Mar.
Article
em En
| MEDLINE
| ID: mdl-25723091
ABSTRACT
The aim is to develop irradiated nanodiamonds (INDs) as a molecularly targeted contrast agent for high-resolution and phenotype-specific detection of breast cancer with photoacoustic (PA) imaging. The surface of acid treated radiation-damaged nanodiamonds was grafted with PEG to improve its stability and circulation time in blood, followed by conjugation to an anti-HER2 peptide with a final nanoparticle size of approximately 92 nm. Immunocompetent mice bearing orthotopic HER2-positive or negative tumors were administered INDs and PA imaged using an 820-nm near-infrared laser. PA images demonstrated that INDs accumulate in tumors and completely delineated the entire tumor within 10 h. HER2 targeting significantly enhanced imaging of HER2-positive tumors. Pathological examination demonstrated INDs are nontoxic. PA technology is adaptable to low-cost bedside medicine, and with new contrast agents described herein, PA can achieve high-resolution (sub-mm) and phenotype-specific monitoring of cancer growth.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Mama
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Neoplasias da Mama
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Meios de Contraste
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Nanodiamantes
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Técnicas Fotoacústicas
Limite:
Animals
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Female
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Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article