Pericytes in the myovascular niche promote post-natal myofiber growth and satellite cell quiescence.
Development
; 142(7): 1242-53, 2015 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-25742797
ABSTRACT
The satellite cells, which serve as adult muscle stem cells, are both located beneath myofiber basement membranes and closely associated with capillary endothelial cells. We observed that 90% of capillaries were associated with pericytes in adult mouse and human muscle. During post-natal growth, newly formed vessels with their neuroglial 2 proteoglycan (NG2)-positive pericytes became progressively associated with the post-natal muscle stem cells, as myofibers increased in size and satellite cells entered into quiescence. In vitro, human muscle-derived pericytes promoted myogenic cell differentiation through insulin-like growth factor 1 (IGF1) and myogenic cell quiescence through angiopoietin 1 (ANGPT1). Diphtheria toxin-induced ablation of muscle pericytes in growing mice led both to myofiber hypotrophy and to impaired establishment of stem cells quiescence. Similar effects were observed following conditional in vivo deletion of pericyte Igf1 and Angpt1 genes, respectively. Our data therefore demonstrate that, by promoting post-natal myogenesis and stem cell quiescence, pericytes play a key role in the microvascular niche of satellite cells.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ciclo Celular
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Fibras Musculares Esqueléticas
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Neovascularização Fisiológica
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Pericitos
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Células Satélites de Músculo Esquelético
Limite:
Adolescent
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Adult
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Animals
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Child
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Child, preschool
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Humans
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Infant
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article