Rouxiella chamberiensis gen. nov., sp. nov., a member of the family Enterobacteriaceae isolated from parenteral nutrition bags.
Int J Syst Evol Microbiol
; 65(Pt 6): 1812-1818, 2015 Jun.
Article
em En
| MEDLINE
| ID: mdl-25747423
ABSTRACT
Parenteral nutrition bags for newborns were found contaminated by a previously undescribed member of the family Enterobacteriaceae. The six isolates studied by rrs gene (encoding 16S rRNA) sequence analysis and multi-locus sequence analysis (MLSA) formed a discrete branch close to the genera Ewingella, Rahnella, Yersinia,Hafnia and Serratia. Phenotypically, the new taxon was distinct from these five genera. The new taxon gave positive results in Voges-Proskauer, Simmons citrate and o-nitrophenyl-ß-galactoside hydrolysis tests; fermented d-glucose, d-mannitol, l-rhamnose, melibiose, l-arabinose and d-xylose; hydrolysed aesculin; and did not ferment maltose, trehalose, raffinose, d-sorbitol, sucrose or cellobiose. Tests for motility, gas production, urease, gelatinase and nitrate reduction were also negative. All isolates failed to grow at 37 °C. The DNA G+C content of strain 130333T was 53 mol%. On the basis of data obtained in this study, the six isolates represent a novel species of a new genus in the family Enterobacteriaceae, named Rouxiella chamberiensis gen. nov., sp. nov. The type strain of the type species is 130333T (â= CIP 110714T = DSM 28324T).
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Filogenia
/
Contaminação de Equipamentos
/
Nutrição Parenteral
/
Enterobacteriaceae
País como assunto:
Europa
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article