Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonarius.
Sci Rep
; 5: 9086, 2015 Mar 13.
Article
em En
| MEDLINE
| ID: mdl-25765923
ABSTRACT
In microorganisms, Ion Torrent sequencing technology has been proved to be useful in whole-genome sequencing of bacterial genomes (5â
Mbp). In our study, for the first time we used this technology to perform a resequencing approach in a whole fungal genome (36â
Mbp), a non-ochratoxin A producing strain of Aspergillus carbonarius. Ochratoxin A (OTA) is a potent nephrotoxin which is found mainly in cereals and their products, but it also occurs in a variety of common foods and beverages. Due to the fact that this strain does not produce OTA, we focused some of the bioinformatics analyses in genes involved in OTA biosynthesis, using a reference genome of an OTA producing strain of the same species. This study revealed that in the atoxigenic strain there is a high accumulation of nonsense and missense mutations in several genes. Importantly, a two fold increase in gene mutation ratio was observed in PKS and NRPS encoding genes which are suggested to be involved in OTA biosynthesis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Aspergillus
/
Genoma Fúngico
/
Análise de Sequência de DNA
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article