[Placental PHLDA2 gene imprinting in patients with pre-eclampsia].
Sichuan Da Xue Xue Bao Yi Xue Ban
; 46(1): 104-7, 128, 2015 Jan.
Article
em Zh
| MEDLINE
| ID: mdl-25807806
OBJECTIVE: To observe the imprinting status of maternally expressed gene pleckstrin homology-like domain, family A, member 2 (PHLDA2) in placental tissues from patients with pre-eclampsia. METHODS: Samples of placental tissues were collected from women with normal pregnancy (n=21) and pre-eclampsia (n=19). We examined two single nucleotide polymorphism (SNPs) which are prone to variation in PHLDA2: the C/T polymorphism in exon 1 and the G/A polymorphism in exon 2, corresponding to rs13390 (PHLDA2-1) and rs1056819 (PHLDA2-2), respectively. DNA PCR-direct sequencing and cDNA RT-PCR-direct sequencing were applied to detect the special-allelic imprinting status of PHLDA2. RESULTS: No heterozygote was found in placental tissues in relation to C/T polymorphism in PHLDA2 exon 1. Differences in heterozygote in relation to G/A polymorphism in PHLDA2 exon 2 were found between pre-eclampsia (4/19) and normal pregnancy(5/21), but without statistical significance. PHLDA2 cDNA from heterozygotes (PHLDA2-2) were all exclusively monoallelically expressed. CONCLUSION: Similargene polymorphism of PHLDA2 (PHLDA2-1 and PHLDA2-2) in placental tissues was found between pre-eclampsia and normal pregnancies. No loss of imprinting (LOI) of PHLDA2 was found in this study.
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Base de dados:
MEDLINE
Assunto principal:
Placenta
/
Pré-Eclâmpsia
/
Proteínas Nucleares
/
Impressão Genômica
Limite:
Female
/
Humans
/
Pregnancy
Idioma:
Zh
Ano de publicação:
2015
Tipo de documento:
Article