A novel hematopoietic progenitor cell mobilization and collection algorithm based on preemptive CD34 enumeration.
Transfusion
; 55(8): 2010-6, 2015 Aug.
Article
em En
| MEDLINE
| ID: mdl-25808119
ABSTRACT
BACKGROUND:
The collection of autologous peripheral blood (PB) stem cells can be challenging in the subgroup of patients deemed "poor mobilizers" with granulocyte-colony-stimulating factor. Plerixafor, a CXCR-4 antagonist, is an alternative mobilizing agent, but is costly, and the optimal mobilization algorithm has yet to be determined. STUDY DESIGN ANDMETHODS:
To address the question we developed a protocol measuring PB CD34 on Day 4 of mobilization. We examined 26 patients before initiating the protocol versus 24 patients after initiation.RESULTS:
Significant differences (p ≤ 0.05) included fewer days of collection (1.25 days vs. 2.42 days), lower total blood volume processed (25.9 L vs. 57.2 L), lower total product volume (324 mL vs. 691 mL), lower RBC content (9 mL vs. 18 mL), and lower granulocyte percentage per collection (35% vs. 11%). There were no significant differences between the two groups in demographics, baseline platelet count, total CD34, or CD34/kg harvested.CONCLUSION:
Use of a protocol to assess PB CD34 1 day before collection allows for preemptive administration of plerixafor to augment mobilization. Subsequently, days of collection and processed blood volume are reduced while there is less RBC and granulocyte contamination in the collected stem cell product.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Algoritmos
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Antígenos CD34
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Mobilização de Células-Tronco Hematopoéticas
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Compostos Heterocíclicos
Tipo de estudo:
Guideline
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Observational_studies
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Prognostic_studies
Limite:
Adult
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Aged
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article