Improving survival of disassociated human embryonic stem cells by mechanical stimulation using acoustic tweezing cytometry.
Biophys J
; 108(6): 1315-1317, 2015 Mar 24.
Article
em En
| MEDLINE
| ID: mdl-25809245
ABSTRACT
Dissociation-induced apoptosis of human embryonic stem cells (hESCs) hampers their large-scale culture. Herein we leveraged the mechanosensitivity of hESCs and employed, to our knowledge, a novel technique, acoustic tweezing cytometry (ATC), for subcellular mechanical stimulation of disassociated single hESCs to improve their survival. By acoustically actuating integrin-bound microbubbles (MBs) to live cells, ATC increased the survival rate and cloning efficiency of hESCs by threefold. A positive correlation was observed between the increased hESC survival rate and total accumulative displacement of integrin-anchored MBs during ATC stimulation. ATC may serve as a promising biocompatible tool to improve hESC culture.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Estimulação Física
/
Técnicas Citológicas
/
Células-Tronco Embrionárias Humanas
Limite:
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article