Phospho-tyrosine dependent protein-protein interaction network.
Mol Syst Biol
; 11(3): 794, 2015 Mar 26.
Article
em En
| MEDLINE
| ID: mdl-25814554
ABSTRACT
Post-translational protein modifications, such as tyrosine phosphorylation, regulate protein-protein interactions (PPIs) critical for signal processing and cellular phenotypes. We extended an established yeast two-hybrid system employing human protein kinases for the analyses of phospho-tyrosine (pY)-dependent PPIs in a direct experimental, large-scale approach. We identified 292 mostly novel pY-dependent PPIs which showed high specificity with respect to kinases and interacting proteins and validated a large fraction in co-immunoprecipitation experiments from mammalian cells. About one-sixth of the interactions are mediated by known linear sequence binding motifs while the majority of pY-PPIs are mediated by other linear epitopes or governed by alternative recognition modes. Network analysis revealed that pY-mediated recognition events are tied to a highly connected protein module dedicated to signaling and cell growth pathways related to cancer. Using binding assays, protein complementation and phenotypic readouts to characterize the pY-dependent interactions of TSPAN2 (tetraspanin 2) and GRB2 or PIK3R3 (p55γ), we exemplarily provide evidence that the two pY-dependent PPIs dictate cellular cancer phenotypes.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Fosfoproteínas
/
Tirosina
/
Mapas de Interação de Proteínas
Limite:
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article