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Potential Neurogenesis of Human Adipose-Derived Stem Cells on Electrospun Catalpol-Loaded Composite Nanofibrous Scaffolds.
Guo, Jian-Hui; Liu, Yang; Lv, Zheng-Jun; Wei, Wen-Juan; Guan, Xin; Guan, Qing-Lin; Leng, Zhi-Qian; Zhao, Jing-Yuan; Miao, Hui; Liu, Jing.
Afiliação
  • Guo JH; Regenerative Medicine Center, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, People's Republic of China.
  • Liu Y; Institute of Integrative Medicine, Dalian Medical University, Dalian, 116044, People's Republic of China.
  • Lv ZJ; Regenerative Medicine Center, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, People's Republic of China.
  • Wei WJ; Institute of Integrative Medicine, Dalian Medical University, Dalian, 116044, People's Republic of China.
  • Guan X; Queen Mary University of London, London, E1 4NS, UK.
  • Guan QL; Regenerative Medicine Center, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, People's Republic of China.
  • Leng ZQ; Regenerative Medicine Center, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, People's Republic of China.
  • Zhao JY; Center Laboratory, Affiliated Zhongshan Hospital of Dalian University, Dalian, 116001, People's Republic of China.
  • Miao H; Regenerative Medicine Center, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, People's Republic of China.
  • Liu J; Regenerative Medicine Center, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, People's Republic of China.
Ann Biomed Eng ; 43(10): 2597-608, 2015 Oct.
Article em En | MEDLINE | ID: mdl-25824369
Catalpol, a natural active ingredient extracted from the traditional Chinese medicine, was verified exhibiting beneficial effects on neural differentiation compared with commonly used chemical inducers by our previous studies. The aim of this study was to evaluate the effects of catalpol-loaded scaffold on guiding neuronal differentiation of human adipose tissue-derived stem cells (hASCs). Fabrication technique of catalpol loading into the electrospun poly(lactic-co-glycolic acid)/multi-walled carbon nanotubes/silk fibroin nanofibrous scaffolds was successfully established. The topographical and mechanical properties of the nanofibers scaffolds were characterized by scanning electron microscopy and tensile instrument, respectively. In vitro catalpol release was studied in phosphate-buffered solution at 37 °C. Immunnocytochemistry, RT-PCR, and western blot assays were performed to estimate hASCs neuronal differentiation, and it was shown that catalpol has significantly upregulated the expressions of ßIII-tubulin and Nissl. Our experiments demonstrated that catalpol, as a traditional Chinese medicine extract, could be encapsulated into composite nanofibers and induce differentiation of hASCs into neural-like cells, which might offer new avenues in nerve regeneration.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células-Tronco / Tecido Adiposo / Alicerces Teciduais / Neurogênese / Nanofibras / Glucosídeos Iridoides Limite: Humans Idioma: En Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células-Tronco / Tecido Adiposo / Alicerces Teciduais / Neurogênese / Nanofibras / Glucosídeos Iridoides Limite: Humans Idioma: En Ano de publicação: 2015 Tipo de documento: Article