The Prevalence of Mixed Helicobacter pylori Infections in Symptomatic and Asymptomatic Subjects in Dhaka, Bangladesh.
Helicobacter
; 20(5): 397-404, 2015 Oct.
Article
em En
| MEDLINE
| ID: mdl-25827337
ABSTRACT
BACKGROUND:
Helicobacter pylori is a highly genetically diverse bacterial species, which can persist in the gastric environment for decades. Recent studies have shown that single infections predominate in developed countries, whereas mixed infections are more prevalent in developing countries. Mixed infections of this bacterium may be important for adaptation to the hostile gastric environment and may facilitate dyspeptic symptoms. MATERIALS ANDMETHODS:
To calculate the prevalence of mixed infections in symptomatic and asymptomatic subjects, 2010 H. pylori isolates collected from 83 symptomatic and 91 asymptomatic subjects from Dhaka, Bangladesh, were analyzed by (i) random amplified polymorphic DNA fingerprinting (RAPD) and (ii) multiplex PCR amplification for cagA and vacA virulence gene alleles.RESULTS:
The overall prevalence of mixed H. pylori infection was 60.15% (77/128), indicating substantial co-colonization in this population. We additionally found that symptomatic subjects (53%) had a significantly higher rate of mixed infection than asymptomatic individuals (36.3%) (p = .016) and that the prevalence of the cagA and vacA and vacA m1/s1 and vacA m2/s1 alleles were higher in subjects with mixed infection.CONCLUSION:
Our findings suggest that an increased diversity of the H. pylori strains in the gastric environment may contribute to the development of disease symptoms.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Variação Genética
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Helicobacter pylori
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Infecções por Helicobacter
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Coinfecção
Tipo de estudo:
Diagnostic_studies
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Prevalence_studies
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Risk_factors_studies
Limite:
Adult
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Child
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Child, preschool
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Female
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Humans
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Male
País como assunto:
Asia
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article