Mucosal vaccination by adenoviruses displaying reovirus sigma 1.
Virology
; 482: 60-6, 2015 Aug.
Article
em En
| MEDLINE
| ID: mdl-25827529
ABSTRACT
We developed adenovirus serotype 5 (Ad5) vectors displaying the sigma 1 protein from reovirus as mucosal vaccines. Ad5-sigma retargets to JAM-1 and sialic acid, but has 40-fold reduced gene delivery when compared to Ad5. While weaker at transduction, Ad5-sigma generates stronger T cell responses than Ad5 when used for mucosal immunization. In this work, new Ad5-fiber-sigma vectors were generated by varying the number of fiber ß-spiral shaft repeats (R) between the fiber tail and sigma. Increasing chimera length led to decreasing insertion of these proteinsAd5 virions. Ad-R3 and R14 vectors effectively targeted JAM-1 in vitro while R20 did not. When wereused to immunize mice by the intranasal route, Ad5-R3-sigma produced higher serum and vaginal antibody responses than Ad5. These data suggest optimized Ad-sigma vectors may be useful vectors for mucosal vaccination.
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Base de dados:
MEDLINE
Assunto principal:
Reoviridae
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Vacinas Virais
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Adenoviridae
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Vacinação
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Proteínas do Capsídeo
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Técnicas de Visualização da Superfície Celular
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article