The frequency natural antisense transcript first promotes, then represses, frequency gene expression via facultative heterochromatin.
Proc Natl Acad Sci U S A
; 112(14): 4357-62, 2015 Apr 07.
Article
em En
| MEDLINE
| ID: mdl-25831497
ABSTRACT
The circadian clock is controlled by a network of interconnected feedback loops that require histone modifications and chromatin remodeling. Long noncoding natural antisense transcripts (NATs) originate from Period in mammals and frequency (frq) in Neurospora. To understand the role of NATs in the clock, we put the frq antisense transcript qrf (frq spelled backwards) under the control of an inducible promoter. Replacing the endogenous qrf promoter altered heterochromatin formation and DNA methylation at frq. In addition, constitutive, low-level induction of qrf caused a dramatic effect on the endogenous rhythm and elevated circadian output. Surprisingly, even though qrf is needed for heterochromatic silencing, induction of qrf initially promoted frq gene expression by creating a more permissible local chromatin environment. The observation that antisense expression can initially promote sense gene expression before silencing via heterochromatin formation at convergent loci is also found when a NAT to hygromycin resistance gene is driven off the endogenous vivid (vvd) promoter in the Δvvd strain. Facultative heterochromatin silencing at frq functions in a parallel pathway to previously characterized VVD-dependent silencing and is needed to establish the appropriate circadian phase. Thus, repression via dicer-independent siRNA-mediated facultative heterochromatin is largely independent of, and occurs alongside, other feedback processes.
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MEDLINE
Assunto principal:
Heterocromatina
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Regulação da Expressão Gênica
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Oligonucleotídeos Antissenso
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Neurospora crassa
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article