Immunomodulatory properties of Tamm-Horsfall glycoprotein (THP) and uromodulin.
Asian Pac J Allergy Immunol
; 33(1): 26-32, 2015 Mar.
Article
em En
| MEDLINE
| ID: mdl-25840631
ABSTRACT
BACKGROUND:
Tamm-Horsfall glycoprotein (THP) and uromodulin are the most abundant glycoproteins in non-pregnant women's/men's and pregnant women's urine, respectively. However, the bioactivities of these glycoproteins are still unclear.OBJECTIVE:
To evaluate the immunomodulatory properties of THP and uromodulin on human peripheral blood mononuclear cells (PBMC)METHODS:
THP and uromodulin isolated with diatomaceous earth filtration were subjected to several bioassays, such as MTS viability assay, immunophenotyping and cytokine analysis.RESULTS:
MTS viability assay and immunophenotyping analysis showed that uromodulin has greater inhibitory activities in suppressing PBMC viability and the percentage of CD4⺠T helper cells and CD8⺠cytotoxic T cells, compared to that of THP. In cytokine analysis, THP tended to induce pro-inflammatory cytokines such as IL-1ß, TNF and Th1 cytokine IFN-γ; while uromodulin only induced IL-1ß and suppressed both Th1 cytokine IFN-γ and Th2 cytokine IL-10.CONCLUSION:
These results demonstrate that uromodulin has greater immunosuppressive activities and lower inductive property in relation to activation of immune cells, which provides a more tolerant environment for the developing fetus.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Linfócitos T Citotóxicos
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Linfócitos T Auxiliares-Indutores
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Uromodulina
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Fatores Imunológicos
Limite:
Adult
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Female
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Humans
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Male
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Pregnancy
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article