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Immunogenicity of human hepatitis B virus P-gene derived proteins.
Weimer, T; Weimer, K; Tu, Z X; Jung, M C; Pape, G R; Will, H.
Afiliação
  • Weimer T; Max-Planck-Institut für Biochemie, Martinsried, Federal Republic of Germany.
J Immunol ; 143(11): 3750-6, 1989 Dec 01.
Article em En | MEDLINE | ID: mdl-2584717
The frequency and specificity of antibodies to P-gene encoded proteins of human hepatitis B virus was tested in sera of acute and chronically infected patients with and without hepatocellular carcinoma (HCC). For antibody detection an immunoprecipitation gel assay was performed with radioactively labeled polypeptides produced by in vitro translation of RNA of different P-gene regions. Thus, five antigenic regions were identified. All anti-P antibody positive sera reacted with carboxy-terminal P-poly-peptides, a subset with polypeptides of the amino-terminal and middle region, and none reacted with P-protein derived from the most sequence variable region. Anti-P antibodies were detected at very high frequency in sera of acute (73%) and chronically infected patients without HCC (87%), but less often in HCC patients (27%). These data indirectly demonstrate the expression of most hepatitis B virus P-gene sequences and the immunogenicity of P-proteins in vivo. Moreover, they establish hepatitis B virus anti-P-antibodies as a frequent serologic marker of infection and identify the carboxy-terminal region of the P-protein(s) as immunodominant.
Assuntos
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Base de dados: MEDLINE Assunto principal: Proteínas do Envelope Viral / Vírus da Hepatite B / Genes Virais Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 1989 Tipo de documento: Article
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Base de dados: MEDLINE Assunto principal: Proteínas do Envelope Viral / Vírus da Hepatite B / Genes Virais Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 1989 Tipo de documento: Article