PFGE protocols to distinguish subspecies of Lactococcus lactis.
Methods Mol Biol
; 1301: 213-24, 2015.
Article
em En
| MEDLINE
| ID: mdl-25862059
Pulsed-field gel electrophoresis (PFGE), developed in the mid-1980s, rapidly became a "gold standard" method for analyzing bacterial chromosomes. Today, although outcompeted in resolution by alternative methods, such as optical mapping, and not applicable for high-throughput analyses, PFGE remains a valuable method for bacterial strain typing. Here, we describe optimized protocols for macrorestriction fingerprinting, characterization of plasmid complements, and gene localization by DNA-DNA hybridization of Lactococcus lactis genomes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Técnicas de Tipagem Bacteriana
/
Eletroforese em Gel de Campo Pulsado
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Lactococcus lactis
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article