Identification of a novel human circulating metabolite of tenofovir disoproxil fumarate with LC-MS/MS.
Bioanalysis
; 7(6): 643-52, 2015.
Article
em En
| MEDLINE
| ID: mdl-25871584
ABSTRACT
BACKGROUND:
Tenofovir disoproxil fumarate (TDF) is an antiretroviral drug used for the treatment of Human Immunodeficiency Virus and Hepatitis B infections.RESULTS:
A metabolite that has previously not been observed in the circulation of humans was detected by LC-MS/MS in early time point plasma samples following administration of TDF to healthy volunteers. The metabolite was identified using a range of LC-MS/MS-based techniques as a monoester of TDF, derived from the partially hydrolyzed bis-ester prodrug. TDF, when spiked into plasma, was observed to degrade first to the putative monoester and subsequently to tenofovir.CONCLUSION:
The presence of this unstable metabolite in some samples has implications for sample collection, handling and storage in studies of tenofovir where serum concentrations are determined.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Análise Química do Sangue
/
Antirretrovirais
/
Espectrometria de Massas em Tandem
/
Tenofovir
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article