Off-response in ASH neurons evoked by CuSO4 requires the TRP channel OSM-9 in Caenorhabditis elegans.
Biochem Biophys Res Commun
; 461(3): 463-8, 2015 Jun 05.
Article
em En
| MEDLINE
| ID: mdl-25871795
ABSTRACT
The off-response of ASH neurons had been overlooked until the microfluidic devices were introduced for in vivo imaging of neuronal activity in Caenorhabditis elegans. The mechanisms of ASH off-response were completely unknown. Here we monitored ASH off-response to CuSO4 stimulation by use of microfluidic device and genetically encoded calcium indicator (GECI) - Case12. We found ASH neurons exhibited a multiphasic response to 10 mM and 50 mM CuSO4 of 30-s stimulation duration. ASH off-responding to Cu(2+) had been dramatically reduced in goa-1, mod-5, trpa-1 and egl-8 mutants. Moreover, in osm-9 mutants ASH off-response was completely eliminated. Neuron-specific rescue of osm-9 in ASH neurons restored the off-response and the normal avoidance behavior in worms.
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MEDLINE
Assunto principal:
Caenorhabditis elegans
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Proteínas de Caenorhabditis elegans
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Canais de Cátion TRPV
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Proteínas do Tecido Nervoso
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Neurônios
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Animals
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En
Ano de publicação:
2015
Tipo de documento:
Article