Nop17 is a key R2TP factor for the assembly and maturation of box C/D snoRNP complex.
BMC Mol Biol
; 16: 7, 2015 Mar 18.
Article
em En
| MEDLINE
| ID: mdl-25888478
ABSTRACT
BACKGROUND:
Box C/D snoRNPs are responsible for rRNA methylation and processing, and are formed by snoRNAs and four conserved proteins, Nop1, Nop56, Nop58 and Snu13. The snoRNP assembly is a stepwise process, involving other protein complexes, among which the R(2)TP and Hsp90 chaperone. Nop17, also known as Pih1, has been shown to be a constituent of the R(2)TP (Rvb1, Rvb2, Tah1, Pih1) and to participate in box C/D snoRNP assembly by its interaction with Nop58. The molecular function of Nop17, however, has not yet been described.RESULTS:
To shed light on the role played by Nop17 in the maturation of snoRNP, here we analyzed the interactions domains of Nop58 - Nop17 - Tah1 and the importance of ATP to the interaction between Nop17 and the ATPase Rvb1/2.CONCLUSIONS:
Based on the results shown here, we propose a model for the assembly of box C/D snoRNP, according to which R(2)TP complex is important for reducing the affinity of Nop58 for snoRNA, and for the binding of the other snoRNP subunits.
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Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Proteínas Nucleares
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Ribonucleoproteínas Nucleolares Pequenas
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Proteínas de Saccharomyces cerevisiae
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article