Increased production of inosine and guanosine by means of metabolic engineering of the purine pathway in Ashbya gossypii.
Microb Cell Fact
; 14: 58, 2015 Apr 17.
Article
em En
| MEDLINE
| ID: mdl-25889888
ABSTRACT
BACKGROUND:
Inosine and guanosine monophosphate nucleotides are convenient sources of the umami flavor, with attributed beneficial health effects that have renewed commercial interest in nucleotide fermentations. Accordingly, several bacterial strains that excrete high levels of inosine and guanosine nucleosides are currently used in the food industry for this purpose.RESULTS:
In the present study, we show that the filamentous fungus Ashbya gossypii, a natural riboflavin overproducer, excretes high amounts of inosine and guanosine nucleosides to the culture medium. Following a rational metabolic engineering approach of the de novo purine nucleotide biosynthetic pathway, we increased the excreted levels of inosine up to 27-fold.CONCLUSIONS:
We generated Ashbya gossypii strains with improved production titers of inosine and guanosine. Our results point to Ashbya gossypii as the first eukaryotic microorganism representing a promising candidate, susceptible to further manipulation, for industrial nucleoside fermentation.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Eremothecium
/
Engenharia Metabólica
/
Guanosina
/
Inosina
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article