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Optimization of amine-rich multilayer thin films for the capture and quantification of prostate-specific antigen.
Polak, Roberta; Bradwell, Grinia M; Gilbert, Jonathan B; Danielsen, Scott; Beppu, Marisa M; Cohen, Robert E; Rubner, Michael F.
Afiliação
  • Polak R; †School of Pharmaceutical Sciences, University of Sao Paulo (USP), Sao Paulo, SP, Brazil.
  • Danielsen S; §Department of Chemical and Biomolecular Engineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States.
  • Beppu MM; ‡School of Chemical Engineering, University of Campinas (UNICAMP), Campinas, SP, Brazil.
Langmuir ; 31(19): 5479-88, 2015 May 19.
Article em En | MEDLINE | ID: mdl-25909861
It is demonstrated that poly(allylamine hydrochloride)/poly(styrenesulfonate) (PAH/SPS) multilayer films can be successfully tailored for the capture and detection of small biomolecules in dilute concentrations. Based on in vitro results, these films could be potentially applied for rapid and high-throughput diagnosis of dilute biomarkers in serum or tissue. PAH presents functional amino groups that can be further reacted with desired chemistries in order to create customizable and specific surfaces for biomolecule capture. A variety of film assembly characteristics were tested (pH, molecular weight of PAH, and ionic strength) to tune the biotinylation and swelling behavior of these films to maximize detection capabilities. The resultant optimized biotinylated PAH/SPS 9.3/9.3 system was utilized in conjunction with quantum dots (Qdots) to capture and detect a dilute biomarker for prostate cancer, prostate-specific antigen (PSA). Compared to previous work, our system presents a good sensitivity for PSA detection within the clinically relevant range of 0.4-100 ng/mL.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Poliaminas / Poliestirenos / Neoplasias da Próstata / Antígeno Prostático Específico Tipo de estudo: Diagnostic_studies Limite: Humans / Male Idioma: En Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Poliaminas / Poliestirenos / Neoplasias da Próstata / Antígeno Prostático Específico Tipo de estudo: Diagnostic_studies Limite: Humans / Male Idioma: En Ano de publicação: 2015 Tipo de documento: Article