Enzymatically degradable poly(ethylene glycol) hydrogels for the 3D culture and release of human embryonic stem cell derived pancreatic precursor cell aggregates.

Amer, Luke D; Holtzinger, Audrey; Keller, Gordon; Mahoney, Melissa J; Bryant, Stephanie J

Amer, Luke D; Holtzinger, Audrey; Keller, Gordon; Mahoney, Melissa J; Bryant, Stephanie J.

Afiliação

Amer LD; Department of Chemical and Biological Engineering, University of Colorado, 3415 Colorado Avenue, Boulder, CO 80303, USA; BioFrontiers Institute, University of Colorado, 3415 Colorado Avenue, Boulder, CO 80303, USA. Electronic address: Luke.Amer@Colorado.EDU.
Holtzinger A; McEwen Centre for Regenerative Medicine, University Health Network, 8-601 TMDT 101 College St., Toronto, ON M5G 1L7, Canada. Electronic address: Aholtzin@uhnres.utoronto.ca.
Keller G; McEwen Centre for Regenerative Medicine, University Health Network, 8-601 TMDT 101 College St., Toronto, ON M5G 1L7, Canada. Electronic address: gkeller@uhnresearch.ca.
Mahoney MJ; Department of Chemical and Biological Engineering, University of Colorado, 3415 Colorado Avenue, Boulder, CO 80303, USA; BioFrontiers Institute, University of Colorado, 3415 Colorado Avenue, Boulder, CO 80303, USA. Electronic address: Melissa.Mahoney@Colorado.EDU.
Bryant SJ; Department of Chemical and Biological Engineering, University of Colorado, 3415 Colorado Avenue, Boulder, CO 80303, USA; BioFrontiers Institute, University of Colorado, 3415 Colorado Avenue, Boulder, CO 80303, USA; Material Science and Engineering Program, University of Colorado, 3415 Colorado Avenu

Acta Biomater ; 22: 103-10, 2015 Aug.

Article em En | MEDLINE | ID: mdl-25913222

Assuntos

Técnicas de Cultura de Células/métodos; Enzimas/metabolismo; Células-Tronco Embrionárias Humanas/citologia; Hidrogéis/química; Pâncreas/citologia; Polietilenoglicóis/química; Sequência de Aminoácidos; Animais; Agregação Celular; Linhagem Celular Tumoral; Tamanho Celular; Sobrevivência Celular; Citometria de Fluxo; Humanos; Camundongos; Microscopia Confocal; Dados de Sequência Molecular; Peptídeos/química; Polimerização; Ratos; Fatores de Transcrição/metabolismo

Palavras-chave

Controlled release; Human embryonic stem cells; Long-term culture platforms; Pancreatic precursor cells; Poly(ethylene glycol) hydrogels

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Pâncreas / Polietilenoglicóis / Técnicas de Cultura de Células / Hidrogéis / Enzimas / Células-Tronco Embrionárias Humanas Limite: Animals / Humans Idioma: En Ano de publicação: 2015 Tipo de documento: Article

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