High-Throughput Dose-Response Measurement Using a Label-Free Microarray-in-Microplate Assay Platform.
Anal Chem
; 87(11): 5640-8, 2015 Jun 02.
Article
em En
| MEDLINE
| ID: mdl-25921700
ABSTRACT
Microarray-based binding assays facilitate the discovery of protein ligands from large collections of small molecules. Hundreds of ligands can be identified, yet only a small portion of them have interfering effects (competitive or noncompetitive) on a specific protein-receptor binding reaction. Further efficient screening of ligands for those with specific modifying effect is needed in order to take the full advantage of throughputs of microarray-based assays for drug discovery. We report a label-free "microarray-in-microplate" assay platform for simultaneous acquisition of at least 32 dose-response curves in a single experiment, each curve having 12 concentration points. When combined with ligand discovery, this makes the microarray-based platform a true high-throughout means of finding inhibitors to specific protein-receptor reactions starting from a large collection of small-molecule libraries.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Bioensaio
/
Análise em Microsséries
/
Relação Dose-Resposta a Droga
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article