Molecular characterization of a Penicillium chrysogenum exo-rhamnogalacturonan lyase that is structurally distinct from other polysaccharide lyase family proteins.

Iwai, Marin; Kawakami, Takuya; Ikemoto, Takeshi; Fujiwara, Daisuke; Takenaka, Shigeo; Nakazawa, Masami; Ueda, Mitsuhiro; Sakamoto, Tatsuji

Iwai, Marin; Kawakami, Takuya; Ikemoto, Takeshi; Fujiwara, Daisuke; Takenaka, Shigeo; Nakazawa, Masami; Ueda, Mitsuhiro; Sakamoto, Tatsuji.

Afiliação

Iwai M; Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, 599-8531, Japan.
Kawakami T; Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, 599-8531, Japan.
Ikemoto T; Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, 599-8531, Japan.
Fujiwara D; Department of Biological Science, Graduate School of Science, Osaka Prefecture University, Sakai, Osaka, 599-8531, Japan.
Takenaka S; Division of Veterinary science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, 598-8531, Japan.
Nakazawa M; Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, 599-8531, Japan.
Ueda M; Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, 599-8531, Japan.
Sakamoto T; Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, 599-8531, Japan. sakamoto@biochem.osakafu-u.ac.jp.

Appl Microbiol Biotechnol ; 99(20): 8515-25, 2015 Oct.

Article em En | MEDLINE | ID: mdl-25921806

Assuntos

Penicillium chrysogenum/enzimologia; Penicillium chrysogenum/genética; Polissacarídeo-Liases/genética; Polissacarídeo-Liases/metabolismo; Clonagem Molecular; DNA Fúngico/química; DNA Fúngico/genética; Escherichia coli/genética; Expressão Gênica; Dados de Sequência Molecular; Proteínas Recombinantes/genética; Proteínas Recombinantes/isolamento & purificação; Proteínas Recombinantes/metabolismo; Análise de Sequência de DNA; Especificidade por Substrato

Palavras-chave

Exo-acting rhamnogalacturonan lyase; Nucleotide sequence; Overexpression; Penicillium chrysogenum; Unsaturated galacturonosyl rhamnose

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Penicillium chrysogenum / Polissacarídeo-Liases Idioma: En Ano de publicação: 2015 Tipo de documento: Article

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Penicillium chrysogenum / Polissacarídeo-Liases Idioma: En Ano de publicação: 2015 Tipo de documento: Article