An aromatic cage is required but not sufficient for binding of Tudor domains of the Polycomblike protein family to H3K36me3.
Epigenetics
; 10(6): 467-73, 2015.
Article
em En
| MEDLINE
| ID: mdl-25923537
ABSTRACT
Polycomblike (Pcl) proteins are important transcriptional regulators and components of the Polycomb Repressive Complex 2 (PRC2). The Tudor domains of human homologs PHF1 and PHF19 have been found to recognize trimethylated lysine 36 of histone H3 (H3K36me3); however, the biological role of Tudor domains of other Pcl proteins remains poorly understood. Here, we characterize the molecular basis underlying histone binding activities of the Tudor domains of the Pcl family. In contrast to a predominant view, we found that the methyl lysine-binding aromatic cage is necessary but not sufficient for recognition of H3K36me3 by these Tudor domains and that a hydrophobic patch, adjacent to the aromatic cage, is also required.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Histona-Lisina N-Metiltransferase
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Epigênese Genética
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Proteínas de Ligação a DNA
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Proteínas do Grupo Polycomb
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Complexo Repressor Polycomb 2
Limite:
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article