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Divergent roles of BECN1 in LC3 lipidation and autophagosomal function.
He, Ruina; Peng, Jingyu; Yuan, Pengfei; Xu, Fang; Wei, Wensheng.
Afiliação
  • He R; a Biodynamic Optical Imaging Center (BIOPIC); Peking-Tsinghua Center for Life Sciences; State Key Laboratory of Protein and Plant Gene Research; School of Life Sciences; Peking University ; Beijing , China.
Autophagy ; 11(5): 740-7, 2015.
Article em En | MEDLINE | ID: mdl-25955014
BECN1/Beclin 1 is regarded as a critical component in the class III phosphatidylinositol 3-kinase (PtdIns3K) complex to trigger autophagy in mammalian cells. Despite its significant role in a number of cellular and physiological processes, the exact function of BECN1 in autophagy remains controversial. Here we created a BECN1 knockout human cell line using the TALEN technique. Surprisingly, the complete loss of BECN1 had little effect on LC3 (MAP1LC3B/LC3B) lipidation, and LC3B puncta resembling autophagosomes by fluorescence microscopy were still evident albeit significantly smaller than those in the wild-type cells. Electron microscopy (EM) analysis revealed that BECN1 deficiency led to malformed autophagosome-like structures containing multiple layers of membranes under amino acid starvation. We further confirmed that the PtdIns3K complex activity and autophagy flux were disrupted in BECN1(-/-) cells. Our results demonstrate the essential role of BECN1 in the functional formation of autophagosomes, but not in LC3B lipidation.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Autofagia / Fagossomos / Proteínas Reguladoras de Apoptose / Lipídeos / Proteínas de Membrana / Proteínas Associadas aos Microtúbulos Limite: Humans Idioma: En Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Autofagia / Fagossomos / Proteínas Reguladoras de Apoptose / Lipídeos / Proteínas de Membrana / Proteínas Associadas aos Microtúbulos Limite: Humans Idioma: En Ano de publicação: 2015 Tipo de documento: Article