Effect in dedicator of cytokinesis 6 (DOCK6) on steroid production in theca cells of follicular cysts.
Biochem Biophys Res Commun
; 462(4): 415-9, 2015 Jul 10.
Article
em En
| MEDLINE
| ID: mdl-25976676
ABSTRACT
Ovarian follicular cysts are one of the most common causes of reproductive failure in mammals. A comparative gene expression approach may aid in elucidating the causes of ovarian cyst disease. In the present study, the differential display technique was used to identify mRNA sequences that accumulate preferentially in theca cells of bovine cystic follicles. Dedicator of cytokinesis 6 (Dock6) expression was observed in the theca cells of cystic follicles. Small interfering RNA (siRNA) knockdown of Dock6 increased progesterone (P4) production and StAR expression in theca cells of high-estrogen follicular cysts, but did not affect androstenedione (A4) production. We propose that Dock6 may be a marker associated with the development of follicular cysts. Additionally, Dock6 may be involved in the development of cystic follicles by suppressing P4 production rather than increasing A4 production in theca cells.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Progesterona
/
Fatores de Troca do Nucleotídeo Guanina
/
Estrogênios
/
Folículo Ovariano
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article