Roles of miRNAs in microcystin-LR-induced Sertoli cell toxicity.
Toxicol Appl Pharmacol
; 287(1): 1-8, 2015 Aug 15.
Article
em En
| MEDLINE
| ID: mdl-25986756
ABSTRACT
Microcystin (MC)-LR, a cyclic heptapeptide, is a potent reproductive system toxin. To understand the molecular mechanisms of MC-induced reproductive system cytotoxicity, we evaluated global changes of miRNA and mRNA expression in mouse Sertoli cells following MC-LR treatment. Our results revealed that the exposure to MC-LR resulted in an altered miRNA expression profile that might be responsible for the modulation of mRNA expression. Bio-functional analysis indicated that the altered genes were involved in specific cellular processes, including cell death and proliferation. Target gene analysis suggested that junction injury in Sertoli cells exposed to MC-LR might be mediated by miRNAs through the regulation of the Sertoli cell-Sertoli cell pathway. Collectively, these findings may enhance our understanding on the modes of action of MC-LR on mouse Sertoli cells as well as the molecular mechanisms underlying the toxicity of MC-LR on the male reproductive system.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Células de Sertoli
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MicroRNAs
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Microcistinas
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article