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Relationship between High-Performance Liquid Chromatography Fingerprints and Uric Acid-Lowering Activities of Cichorium intybus L.
Zhu, Chun-Sheng; Zhang, Bing; Lin, Zhi-Jian; Wang, Xue-Jie; Zhou, Yue; Sun, Xiao-Xia; Xiao, Ming-Liang.
Afiliação
  • Zhu CS; School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. 20130931518@bucm.edu.cn.
  • Zhang B; School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. zhangb@bucm.edu.cn.
  • Lin ZJ; School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. linzhijian@bucm.edu.cn.
  • Wang XJ; School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. wangxuejie09@sina.com.
  • Zhou Y; School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. Zhouyuewolf@163.com.
  • Sun XX; School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. Sunxiaoxia321@sina.com.
  • Xiao ML; School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. Shirley0601211@126.com.
Molecules ; 20(5): 9455-67, 2015 May 22.
Article em En | MEDLINE | ID: mdl-26007193
This study aimed to explore the spectrum-effect relationships between high-performance liquid chromatography fingerprints and the uric acid-lowering activities of chicory. Chemical fingerprints of chicory samples from ten different sources were determined by high-performance liquid chromatography, and then investigated by similarity analysis and hierarchical clustering analysis. Pharmacodynamics experiments were conducted in animals to obtain the uric acid-lowering activity information of each chicory sample. The spectrum-effect relationships between chemical fingerprints and the uric acid-lowering activities of chicory were established by canonical correlation analysis. The structures of potential effective peaks were identified by liquid chromatography with tandem mass spectrometry. The results showed that a close correlation existed between the spectrum and effect of chicory. Aesculin, chlorogenic acid, chicoric acid, isochlorogenic acid A/B/C and 13,14-seco-stigma5(6),14(15)-diene-3α-ol might be the main effective constituents. This work provides a general model of the combination of high-performance liquid chromatography and uric acid-lowering activities to study the spectrum-effect relationships of chicory, which can be used to discover the principle components responsible for the bioactivity.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ácido Úrico / Extratos Vegetais / Cichorium intybus / Hiperuricemia Limite: Animals Idioma: En Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ácido Úrico / Extratos Vegetais / Cichorium intybus / Hiperuricemia Limite: Animals Idioma: En Ano de publicação: 2015 Tipo de documento: Article