OsWS1 involved in cuticular wax biosynthesis is regulated by osa-miR1848.
Plant Cell Environ
; 38(12): 2662-73, 2015 Dec.
Article
em En
| MEDLINE
| ID: mdl-26012744
ABSTRACT
Cuticular wax forms a hydrophobic layer covering aerial plant organs and acting as a protective barrier against biotic and abiotic stresses. Compared with well-known wax biosynthetic pathway, molecular regulation of wax biosynthesis is less known. Here, we show that rice OsWS1, a member of the membrane-bound O-acyl transferase gene family, involved in wax biosynthesis and was regulated by an osa-miR1848. OsWS1-tagged green fluorescent protein localized to the endoplasmic reticulum (ER). Compared with wild-type rice, OsWS1 overexpression plants displayed a 3% increase in total wax, especially a 35% increase in very long-chain fatty acids, denser wax papillae around the stoma, more cuticular wax crystals formed on leaf and stem surfaces, pollen coats were thicker and more seedlings survived after water-deficit treatment. In contrast, OsWS1-RNAi and osa-miR1848 overexpression plants exhibited opposing changes. Gene expression analysis showed that overexpression of osa-miR1848 down-regulated OsWS1 transcripts; furthermore, expression profiles of OsWS1 and osa-miR1848 were inversely correlated in the leaf, panicle and stem, and upon water-deficit treatment. These results suggest that OsWS1 is regulated by osa-miR1848 and participates in cuticular wax formation.
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Base de dados:
MEDLINE
Assunto principal:
Oryza
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Ceras
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Aciltransferases
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Regulação Enzimológica da Expressão Gênica
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MicroRNAs
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article