Rapid quick, easy, cheap, effective, rugged, and safe extraction with novel phospholipid cleanup: A streamlined ultra high performance liquid chromatography with ultraviolet detection approach for screening polycyclic aromatic hydrocarbons in avian blood cells and plasma.
J Sep Sci
; 38(15): 2677-83, 2015 Aug.
Article
em En
| MEDLINE
| ID: mdl-26013030
ABSTRACT
A streamlined method has been developed for the isolation and analysis of polycyclic aromatic hydrocarbons in avian blood cells and plasma utilizing quick, easy, cheap, effective, rugged, and safe extraction in combination with novel phospholipid cleanup technology. A variety of traditional extraction and cleanup techniques have been employed in the preparation and analysis of polycyclic aromatic hydrocarbonsin a variety of matrices; liquid-liquid partitioning, solid-phase extractions, gel permeation chromatography, and column chromatography are all effective techniques, however they are laborious and time consuming processes that require large amounts of solvent. Using quick, easy, cheap, effective, rugged, and safe extraction coupled with phospholipid cleanup, samples can be quickly screened while maintaining high throughput and sensitivity. With a liquid chromatography approach, analysis times may be kept short at 16 min while maintaining high analyte recovery. Recoveries in quality control samples ranged from 70 to 109%, with average surrogate recoveries of 80.6 ± 1.10%. The result of using a quick, easy, cheap, effective, rugged, and safe extraction approach in conjunction with phospholipid cleanup is a methodology that significantly reduces sample preparation time and solvent use while maintaining high sensitivity and reproducibility.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fosfolipídeos
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Hidrocarbonetos Policíclicos Aromáticos
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Espectrofotometria Ultravioleta
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Aves
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Cromatografia Líquida de Alta Pressão
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article