Efficient genetic encoding of phosphoserine and its nonhydrolyzable analog.
Nat Chem Biol
; 11(7): 496-503, 2015 Jul.
Article
em En
| MEDLINE
| ID: mdl-26030730
Serine phosphorylation is a key post-translational modification that regulates diverse biological processes. Powerful analytical methods have identified thousands of phosphorylation sites, but many of their functions remain to be deciphered. A key to understanding the function of protein phosphorylation is access to phosphorylated proteins, but this is often challenging or impossible. Here we evolve an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair that directs the efficient incorporation of phosphoserine (pSer (1)) into recombinant proteins in Escherichia coli. Moreover, combining the orthogonal pair with a metabolically engineered E. coli enables the site-specific incorporation of a nonhydrolyzable analog of pSer. Our approach enables quantitative decoding of the amber stop codon as pSer, and we purify, with yields of several milligrams per liter of culture, proteins bearing biologically relevant phosphorylations that were previously challenging or impossible to access--including phosphorylated ubiquitin and the kinase Nek7, which is synthetically activated by a genetically encoded phosphorylation in its activation loop.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fosfosserina
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Regulação Bacteriana da Expressão Gênica
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Processamento de Proteína Pós-Traducional
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Proteínas de Escherichia coli
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Escherichia coli
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Aminoacil-tRNA Sintetases
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article