Possible role of cortactin phosphorylation by protein kinase Cα in actin-bundle formation at growth cone.
Biol Cell
; 107(9): 319-30, 2015 Sep.
Article
em En
| MEDLINE
| ID: mdl-26033110
ABSTRACT
BACKGROUND INFORMATION Cortactin contributes to growth cone morphogenesis by forming with dynamin, ring-shaped complexes that mechanically bundle and stabilise F-actin. However, the regulatory mechanism of cortactin action is poorly understood. RESULTS:
Immunofluorescence microscopy revealed that protein kinase C (PKC) α colocalises with cortactin at growth cone filopodia in SH-SY5Y neuroblastoma cells. PKC activation by phorbol 12-myristate 13-acetate causes cortactin phosphorylation, filopodial retraction and F-actin-bundle loss. Moreover, PKCα directly phosphorylates cortactin in vitro at S135/T145/S172, mitigating both cortactin's actin-binding and actin-crosslinking activity, whereas cellular expression of a phosphorylation-mimetic cortactin mutant hinders filopodial formation with a significant decrease of actin bundles.CONCLUSIONS:
Our results indicate that PKC-mediated cortactin phosphorylation might be implicated in the maintenance of growth cone.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Citoesqueleto de Actina
/
Actinas
/
Cones de Crescimento
/
Cortactina
/
Proteína Quinase C-alfa
Limite:
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article