Establishment and cryopreservation of a giant panda skeletal muscle-derived cell line.
Biopreserv Biobank
; 13(3): 195-9, 2015 Jun.
Article
em En
| MEDLINE
| ID: mdl-26035009
ABSTRACT
The giant panda Ailuropoda melanoleuca is an endangered species and is a symbol for wildlife conservation. Although efforts have been made to protect this rare and endangered species through breeding and conservative biology, the long-term preservation of giant panda genome resources (gametes, tissues, organs, genomic libraries, etc.) is still a practical option. In this study, the giant panda skeletal muscle-derived cell line was successfully established via primary explants culture and cryopreservation techniques. The population doubling time of giant panda skeletal cells was approximately 33.8 h, and this population maintained a high cell viability before and after cryopreservation (95.6% and 90.7%, respectively). The two skeletal muscle-specific genes SMYD1 and MYF6 were expressed and detected by RT-PCR in the giant panda skeletal muscle-derived cell line. Karyotyping analysis revealed that the frequencies of giant panda skeletal muscle cells showing a chromosome number of 2n=42 ranged from 90.6â¼94.2%. Thus, the giant panda skeletal muscle-derived cell line provides a vital resource and material platform for further studies and is likely to be useful for the protection of this rare and endangered species.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ursidae
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Criopreservação
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Músculo Esquelético
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article