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MicroRNA-103/107 Regulate Programmed Necrosis and Myocardial Ischemia/Reperfusion Injury Through Targeting FADD.
Wang, Jian-Xun; Zhang, Xiao-Jie; Li, Qian; Wang, Kun; Wang, Yin; Jiao, Jian-Qin; Feng, Chang; Teng, Sun; Zhou, Lu-Yu; Gong, Ying; Zhou, Zhi-Xia; Liu, Jia; Wang, Jian-Ling; Li, Pei-feng.
Afiliação
  • Wang JX; From the Institute for Translational Medicine, College of Medicine, Qingdao University, Qingdao, China (J.-X.W., K.W., Y.W., S.T., L.-Y.Z., Y.G., Z.-X.Z., J.L., J.-L.W., P.-f.L.); and State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, B
  • Zhang XJ; From the Institute for Translational Medicine, College of Medicine, Qingdao University, Qingdao, China (J.-X.W., K.W., Y.W., S.T., L.-Y.Z., Y.G., Z.-X.Z., J.L., J.-L.W., P.-f.L.); and State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, B
  • Li Q; From the Institute for Translational Medicine, College of Medicine, Qingdao University, Qingdao, China (J.-X.W., K.W., Y.W., S.T., L.-Y.Z., Y.G., Z.-X.Z., J.L., J.-L.W., P.-f.L.); and State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, B
  • Wang K; From the Institute for Translational Medicine, College of Medicine, Qingdao University, Qingdao, China (J.-X.W., K.W., Y.W., S.T., L.-Y.Z., Y.G., Z.-X.Z., J.L., J.-L.W., P.-f.L.); and State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, B
  • Wang Y; From the Institute for Translational Medicine, College of Medicine, Qingdao University, Qingdao, China (J.-X.W., K.W., Y.W., S.T., L.-Y.Z., Y.G., Z.-X.Z., J.L., J.-L.W., P.-f.L.); and State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, B
  • Jiao JQ; From the Institute for Translational Medicine, College of Medicine, Qingdao University, Qingdao, China (J.-X.W., K.W., Y.W., S.T., L.-Y.Z., Y.G., Z.-X.Z., J.L., J.-L.W., P.-f.L.); and State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, B
  • Feng C; From the Institute for Translational Medicine, College of Medicine, Qingdao University, Qingdao, China (J.-X.W., K.W., Y.W., S.T., L.-Y.Z., Y.G., Z.-X.Z., J.L., J.-L.W., P.-f.L.); and State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, B
  • Teng S; From the Institute for Translational Medicine, College of Medicine, Qingdao University, Qingdao, China (J.-X.W., K.W., Y.W., S.T., L.-Y.Z., Y.G., Z.-X.Z., J.L., J.-L.W., P.-f.L.); and State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, B
  • Zhou LY; From the Institute for Translational Medicine, College of Medicine, Qingdao University, Qingdao, China (J.-X.W., K.W., Y.W., S.T., L.-Y.Z., Y.G., Z.-X.Z., J.L., J.-L.W., P.-f.L.); and State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, B
  • Gong Y; From the Institute for Translational Medicine, College of Medicine, Qingdao University, Qingdao, China (J.-X.W., K.W., Y.W., S.T., L.-Y.Z., Y.G., Z.-X.Z., J.L., J.-L.W., P.-f.L.); and State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, B
  • Zhou ZX; From the Institute for Translational Medicine, College of Medicine, Qingdao University, Qingdao, China (J.-X.W., K.W., Y.W., S.T., L.-Y.Z., Y.G., Z.-X.Z., J.L., J.-L.W., P.-f.L.); and State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, B
  • Liu J; From the Institute for Translational Medicine, College of Medicine, Qingdao University, Qingdao, China (J.-X.W., K.W., Y.W., S.T., L.-Y.Z., Y.G., Z.-X.Z., J.L., J.-L.W., P.-f.L.); and State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, B
  • Wang JL; From the Institute for Translational Medicine, College of Medicine, Qingdao University, Qingdao, China (J.-X.W., K.W., Y.W., S.T., L.-Y.Z., Y.G., Z.-X.Z., J.L., J.-L.W., P.-f.L.); and State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, B
  • Li PF; From the Institute for Translational Medicine, College of Medicine, Qingdao University, Qingdao, China (J.-X.W., K.W., Y.W., S.T., L.-Y.Z., Y.G., Z.-X.Z., J.L., J.-L.W., P.-f.L.); and State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, B
Circ Res ; 117(4): 352-63, 2015 Jul 31.
Article em En | MEDLINE | ID: mdl-26038570
ABSTRACT
RATIONALE Necrosis is one of the main forms of cardiomyocyte death in heart disease. Recent studies have demonstrated that certain types of necrosis are regulated and programmed dependent on the activation of receptor-interacting serine/threonine-protein kinase (RIPK) 1 and 3 which may be negatively regulated by Fas-associated protein with death domain (FADD). In addition, microRNAs and long noncoding RNAs have been shown to play important roles in various biological processes recently.

OBJECTIVE:

The purpose of this study was to test the hypothesis that microRNA-103/107 and H19 can participate in the regulation of RIPK1- and RIPK3-dependent necrosis in fetal cardiomyocyte-derived H9c2 cells and myocardial infarction through targeting FADD. METHODS AND

RESULTS:

Our results show that FADD participates in H2O2-induced necrosis by influencing the formation of RIPK1 and RIPK3 complexes in H9c2 cells. We further demonstrate that miR-103/107 target FADD directly. Knockdown of miR-103/107 antagonizes necrosis in the cellular model and also myocardial infarction in a mouse ischemia/reperfusion model. The miR-103/107-FADD pathway does not participate in tumor necrosis factor-α-induced necrosis. In exploring the molecular mechanism by which miR-103/107 are regulated, we show that long noncoding RNA H19 directly binds to miR-103/107 and regulates FADD expression and necrosis.

CONCLUSIONS:

Our results reveal a novel myocardial necrosis regulation model, which is composed of H19, miR-103/107, and FADD. Modulation of their levels may provide a new approach for preventing myocardial necrosis.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Traumatismo por Reperfusão Miocárdica / Miócitos Cardíacos / MicroRNAs / Proteína de Domínio de Morte Associada a Fas / Infarto do Miocárdio Tipo de estudo: Prognostic_studies Limite: Animals / Humans / Male Idioma: En Ano de publicação: 2015 Tipo de documento: Article
Texto completo
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PubMed Links
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Traumatismo por Reperfusão Miocárdica / Miócitos Cardíacos / MicroRNAs / Proteína de Domínio de Morte Associada a Fas / Infarto do Miocárdio Tipo de estudo: Prognostic_studies Limite: Animals / Humans / Male Idioma: En Ano de publicação: 2015 Tipo de documento: Article