Polymerase Chain Reaction on a Viral Nanoparticle.
ACS Synth Biol
; 4(12): 1316-25, 2015 Dec 18.
Article
em En
| MEDLINE
| ID: mdl-26046486
ABSTRACT
The field of synthetic biology includes studies that aim to develop new materials and devices from biomolecules. In recent years, much work has been carried out using a range of biomolecular chassis including α-helical coiled coils, ß-sheet amyloids and even viral particles. In this work, we show how hybrid bionanoparticles can be produced from a viral M13 bacteriophage scaffold through conjugation with DNA primers that can template a polymerase chain reaction (PCR). This unprecedented example of a PCR on a virus particle has been studied by flow aligned linear dichroism spectroscopy, which gives information on the structure of the product as well as a new protototype methodology for DNA detection. We propose that this demonstration of PCR on the surface of a bionanoparticle is a useful addition to ways in which hybrid assemblies may be constructed using synthetic biology.
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Base de dados:
MEDLINE
Assunto principal:
Reação em Cadeia da Polimerase
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Bacteriófago M13
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Primers do DNA
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Nanopartículas
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article