Clonal analysis of individual human embryonic stem cell differentiation patterns in microfluidic cultures.
Biotechnol J
; 10(10): 1546-54, 2015 Oct.
Article
em En
| MEDLINE
| ID: mdl-26059045
ABSTRACT
Heterogeneity in the clonal outputs of individual human embryonic stem cells (hESCs) confounds analysis of their properties in studies of bulk populations and how to manipulate them for clinical applications. To circumvent this problem we developed a microfluidic device that supports the robust generation of colonies derived from single ESCs. This microfluidic system contains 160 individually addressable chambers equipped for perfusion culture of individual hESCs that could be shown to match the growth rates, marker expression and colony morphologies obtained in conventional cultures. Use of this microfluidic device to analyze the clonal growth kinetics of multiple individual hESCs induced to differentiation revealed variable shifts in the growth rate, area per cell and expression of OCT4 in the progeny of individual hESCs. Interestingly, low OCT4 expression, a slower growth rate and low nuclear to cytoplasmic ratios were found to be correlated responses. This study demonstrates how microfluidic systems can be used to enable large scale live-cell imaging of isolated hESCs exposed to changing culture conditions, to examine how different aspects of their variable responses are correlated.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Técnicas de Cultura de Células
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Células-Tronco Pluripotentes
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Técnicas Analíticas Microfluídicas
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Células-Tronco Embrionárias Humanas
Limite:
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article