Deconvolution-free Subcellular Imaging with Axially Swept Light Sheet Microscopy.
Biophys J
; 108(12): 2807-15, 2015 Jun 16.
Article
em En
| MEDLINE
| ID: mdl-26083920
ABSTRACT
The use of propagation invariant Bessel beams has enabled high-resolution subcellular light sheet fluorescence microscopy. However, the energy within the concentric side lobe structure of Bessel beams increases significantly with propagation length, generating unwanted out-of-focus fluorescence that enforces practical limits on the imaging field of view size. Here, we present a light sheet fluorescence microscope that achieves 390 nm isotropic resolution and high optical sectioning strength (i.e., out-of-focus blur is strongly suppressed) over large field of views, without the need for structured illumination or deconvolution-based postprocessing. We demonstrate simultaneous dual-color, high-contrast, and high-dynamic-range time-lapse imaging of migrating cells in complex three-dimensional microenvironments, three-dimensional tracking of clathrin-coated pits, and long-term imaging spanning >10 h and encompassing >2600 time points.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Epitélio Pigmentado da Retina
/
Imagem com Lapso de Tempo
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Imagem Óptica
Limite:
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article