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Color-coding cancer and stromal cells with genetic reporters in a patient-derived orthotopic xenograft (PDOX) model of pancreatic cancer enhances fluorescence-guided surgery.
Yano, S; Hiroshima, Y; Maawy, A; Kishimoto, H; Suetsugu, A; Miwa, S; Toneri, M; Yamamoto, M; Katz, M H G; Fleming, J B; Urata, Y; Tazawa, H; Kagawa, S; Bouvet, M; Fujiwara, T; Hoffman, R M.
Afiliação
  • Yano S; 1] AntiCancer, San Diego, CA, USA [2] Department of Surgery, University of California San Diego, San Diego, CA, USA [3] Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
  • Hiroshima Y; 1] AntiCancer, San Diego, CA, USA [2] Department of Surgery, University of California San Diego, San Diego, CA, USA.
  • Maawy A; Department of Surgery, University of California San Diego, San Diego, CA, USA.
  • Kishimoto H; Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
  • Suetsugu A; Department of Gastroenterology, Gifu University Graduate School of Medicine, Gifu, Japan.
  • Miwa S; 1] AntiCancer, San Diego, CA, USA [2] Department of Surgery, University of California San Diego, San Diego, CA, USA.
  • Toneri M; 1] AntiCancer, San Diego, CA, USA [2] Department of Surgery, University of California San Diego, San Diego, CA, USA.
  • Yamamoto M; 1] AntiCancer, San Diego, CA, USA [2] Department of Surgery, University of California San Diego, San Diego, CA, USA.
  • Katz MH; Department of Surgical Oncoloy, MD Anderson Cancer Center, University of Texas, Houston, TX, USA.
  • Fleming JB; Department of Surgical Oncoloy, MD Anderson Cancer Center, University of Texas, Houston, TX, USA.
  • Urata Y; Oncolys BioPharm, Tokyo, Japan.
  • Tazawa H; Center for Innovative Clinical Medicine, Okayama University Hospital, Okayama, Japan.
  • Kagawa S; Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
  • Bouvet M; Department of Surgery, University of California San Diego, San Diego, CA, USA.
  • Fujiwara T; Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
  • Hoffman RM; 1] AntiCancer, San Diego, CA, USA [2] Department of Surgery, University of California San Diego, San Diego, CA, USA.
Cancer Gene Ther ; 22(7): 344-50, 2015 Jul.
Article em En | MEDLINE | ID: mdl-26088297
ABSTRACT
Precise fluorescence-guided surgery (FGS) for pancreatic cancer has the potential to greatly improve the outcome in this recalcitrant disease. To achieve this goal, we have used genetic reporters to color code cancer and stroma cells in a patient-derived orthotopic xenograft (PDOX) model. The telomerase-dependent green fluorescent protein (GFP)-containing adenovirus OBP-401 was used to label the cancer cells of a pancreatic cancer PDOX. The PDOX was previously grown in a red fluorescent protein (RFP) transgenic mouse that stably labeled the PDOX stroma cells bright red. The color-coded PDOX model enabled FGS to completely resect the pancreatic tumors including stroma. Dual-colored FGS significantly prevented local recurrence, which bright-light surgery or single-color FGS could not. FGS, with color-coded cancer and stroma cells has important potential for improving the outcome of recalcitrant-cancer surgery.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias Pancreáticas / Cirurgia Assistida por Computador / Recidiva Local de Neoplasia Limite: Animals Idioma: En Ano de publicação: 2015 Tipo de documento: Article
Texto completo
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias Pancreáticas / Cirurgia Assistida por Computador / Recidiva Local de Neoplasia Limite: Animals Idioma: En Ano de publicação: 2015 Tipo de documento: Article