Quantitative proteomics using SILAC: Principles, applications, and developments.
Proteomics
; 15(18): 3175-92, 2015 Sep.
Article
em En
| MEDLINE
| ID: mdl-26097186
ABSTRACT
SILAC is based on direct addition of selected stable isotope amino acids into the cell culture medium, allowing superior quantitative analysis of the cellular proteome compared to other labeling methods. The great advantages of SILAC lie in its straight-forward implementation, quantitative accuracy, and reproducibility over chemical labeling or label-free quantification strategies, favoring its adoption for proteomic research. SILAC has been widely applied to characterize the proteomic changes between different biological samples, to investigate dynamic changes of protein PTMs, to distinguish specific interacting proteins in interaction proteomic analysis, and to analyze protein turnover in the proteome-wide scale. The present review summarizes the principles of SILAC technology, its applications in biological research, and the present state of this technology.
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Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas
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Proteômica
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Marcação por Isótopo
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article