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MicroRNA396-Targeted SHORT VEGETATIVE PHASE Is Required to Repress Flowering and Is Related to the Development of Abnormal Flower Symptoms by the Phyllody Symptoms1 Effector.
Yang, Chiao-Yin; Huang, Yu-Hsin; Lin, Chan-Pin; Lin, Yen-Yu; Hsu, Hao-Chun; Wang, Chun-Neng; Liu, Li-Yu Daisy; Shen, Bing-Nan; Lin, Shih-Shun.
Afiliação
  • Yang CY; Institute of Biotechnology (C.-Y.Y., Y.-H.H., C.-P.L., Y.-Y.L., B.-N.S., S.-S.L.), Genome and Systems Biology Degree Program (S.-S.L.), Department of Plant Pathology and Microbiology (C.-Y.Y., Y.-H.H., C.-P.L., Y.-Y.L.), Institute of Ecology and Evolutionary Biology, Department of Life Science (H.-C
  • Huang YH; Institute of Biotechnology (C.-Y.Y., Y.-H.H., C.-P.L., Y.-Y.L., B.-N.S., S.-S.L.), Genome and Systems Biology Degree Program (S.-S.L.), Department of Plant Pathology and Microbiology (C.-Y.Y., Y.-H.H., C.-P.L., Y.-Y.L.), Institute of Ecology and Evolutionary Biology, Department of Life Science (H.-C
  • Lin CP; Institute of Biotechnology (C.-Y.Y., Y.-H.H., C.-P.L., Y.-Y.L., B.-N.S., S.-S.L.), Genome and Systems Biology Degree Program (S.-S.L.), Department of Plant Pathology and Microbiology (C.-Y.Y., Y.-H.H., C.-P.L., Y.-Y.L.), Institute of Ecology and Evolutionary Biology, Department of Life Science (H.-C
  • Lin YY; Institute of Biotechnology (C.-Y.Y., Y.-H.H., C.-P.L., Y.-Y.L., B.-N.S., S.-S.L.), Genome and Systems Biology Degree Program (S.-S.L.), Department of Plant Pathology and Microbiology (C.-Y.Y., Y.-H.H., C.-P.L., Y.-Y.L.), Institute of Ecology and Evolutionary Biology, Department of Life Science (H.-C
  • Hsu HC; Institute of Biotechnology (C.-Y.Y., Y.-H.H., C.-P.L., Y.-Y.L., B.-N.S., S.-S.L.), Genome and Systems Biology Degree Program (S.-S.L.), Department of Plant Pathology and Microbiology (C.-Y.Y., Y.-H.H., C.-P.L., Y.-Y.L.), Institute of Ecology and Evolutionary Biology, Department of Life Science (H.-C
  • Wang CN; Institute of Biotechnology (C.-Y.Y., Y.-H.H., C.-P.L., Y.-Y.L., B.-N.S., S.-S.L.), Genome and Systems Biology Degree Program (S.-S.L.), Department of Plant Pathology and Microbiology (C.-Y.Y., Y.-H.H., C.-P.L., Y.-Y.L.), Institute of Ecology and Evolutionary Biology, Department of Life Science (H.-C
  • Liu LY; Institute of Biotechnology (C.-Y.Y., Y.-H.H., C.-P.L., Y.-Y.L., B.-N.S., S.-S.L.), Genome and Systems Biology Degree Program (S.-S.L.), Department of Plant Pathology and Microbiology (C.-Y.Y., Y.-H.H., C.-P.L., Y.-Y.L.), Institute of Ecology and Evolutionary Biology, Department of Life Science (H.-C
  • Shen BN; Institute of Biotechnology (C.-Y.Y., Y.-H.H., C.-P.L., Y.-Y.L., B.-N.S., S.-S.L.), Genome and Systems Biology Degree Program (S.-S.L.), Department of Plant Pathology and Microbiology (C.-Y.Y., Y.-H.H., C.-P.L., Y.-Y.L.), Institute of Ecology and Evolutionary Biology, Department of Life Science (H.-C
  • Lin SS; Institute of Biotechnology (C.-Y.Y., Y.-H.H., C.-P.L., Y.-Y.L., B.-N.S., S.-S.L.), Genome and Systems Biology Degree Program (S.-S.L.), Department of Plant Pathology and Microbiology (C.-Y.Y., Y.-H.H., C.-P.L., Y.-Y.L.), Institute of Ecology and Evolutionary Biology, Department of Life Science (H.-C
Plant Physiol ; 168(4): 1702-16, 2015 Aug.
Article em En | MEDLINE | ID: mdl-26103992
ABSTRACT
Leafy flowers are the major symptoms of peanut witches' broom (PnWB) phytoplasma infection in Catharanthus roseus. The orthologs of the phyllody symptoms1 (PHYL1) effector of PnWB from other species of phytoplasma can trigger the proteasomal degradation of several MADS box transcription factors, resulting in leafy flower formation. In contrast, the flowering negative regulator gene SHORT VEGETATIVE PHASE (SVP) was up-regulated in PnWB-infected C. roseus plants, but most microRNA (miRNA) genes had repressed expression. Coincidentally, transgenic Arabidopsis (Arabidopsis thaliana) plants expressing the PHYL1 gene of PnWB (PHYL1 plants), which show leafy flower phenotypes, up-regulate SVP of Arabidopsis (AtSVP) but repress a putative regulatory miRNA of AtSVP, miR396. However, the mechanism by which PHYL1 regulates AtSVP and miR396 is unknown, and the evidence of miR396-mediated AtSVP degradation is lacking. Here, we show that miR396 triggers AtSVP messenger RNA (mRNA) decay using genetic approaches, a reporter assay, and high-throughput degradome profiles. Genetic evidence indicates that PHYL1 plants and atmir396a-1 mutants have higher AtSVP accumulation, whereas the transgenic plants overexpressing MIR396 display lower AtSVP expression. The reporter assay indicated that target-site mutation results in decreasing the miR396-mediated repression efficiency. Moreover, degradome profiles revealed that miR396 triggers AtSVP mRNA decay rather than miRNA-mediated cleavage, implying that AtSVP caused miR396-mediated translation inhibition. We hypothesize that PHYL1 directly or indirectly interferes with miR396-mediated AtSVP mRNA decay and synergizes with other effects (e.g. MADS box transcription factor degradation), resulting in abnormal flower formation. We anticipate our findings to be a starting point for studying the posttranscriptional regulation of PHYL1 effectors in symptom development.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Arabidopsis / Proteínas de Arabidopsis / Flores / MicroRNAs Tipo de estudo: Diagnostic_studies Idioma: En Ano de publicação: 2015 Tipo de documento: Article
Texto completo
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Arabidopsis / Proteínas de Arabidopsis / Flores / MicroRNAs Tipo de estudo: Diagnostic_studies Idioma: En Ano de publicação: 2015 Tipo de documento: Article