[Sodium Ferulate Attenuates Oxidative Stress Induced Inflammation via Suppressing NALP3 and NF-κB Signal Pathway].

OBJECTIVE: To study the effects of sodium ferulate on inflammation in human lung epithelial cells (A549) under oxidative stress and itsinfluence onthe expression of inflammasome NACHT-PYD-containing protein 3 (NALP3) and nuclear factor kappa B (NF-κB). METHODS: Human lung epithelial cells A549 cultured in vitro were divided into 4 groups, including control group, H2O2 (100µmol/L) stress group, NF-κB blockers group (PDTC 100 µmol/L+ H2O2 100 µmol/L), sodium ferulate (SF) intervention group (SF 400µg/mL+ H2O2 10µmol/L). The expression of NALP3,IκBα protein were evaluated by Western blot, while mRNA levels of NALP3, NF-κB (P65) were measured by qRT-PCR. The level of interleukin-1beta (ILß1) were detected by ELISA. RESULTS: H2O2 not only increased the mRNA and protein expression levels of NALP3, but also enhanced the secretion of ILß1p in human lung epithelial cells A549 (P<0. 05) when compared with control group. NF-κB blockers PDTC and sodium ferulateresisted the effects of H2O2 on A549 cells, that decreased the mRNA and protein expression of NALP3 and the mRNA expression of NF-κB (P65), reduced the degeneration of IκBα and the secretion of IL-1ß (P<0. 05) when compared to H2O2 stress group. CONCLUSION: SF may reduce the expression of NALP3 and IL-1ß by inhibiting NF-κB, so as to reduce the inflammation caused by oxidative stress.