Fast two-dimensional super-resolution image reconstruction algorithm for ultra-high emitter density.
Opt Lett
; 40(13): 2989-92, 2015 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-26125349
ABSTRACT
Single-molecule localization microscopy achieves sub-diffraction-limit resolution by localizing a sparse subset of stochastically activated emitters in each frame. Its temporal resolution is limited by the maximal emitter density that can be handled by the image reconstruction algorithms. Multiple algorithms have been developed to accurately locate the emitters even when they have significant overlaps. Currently, compressive-sensing-based algorithm (CSSTORM) achieves the highest emitter density. However, CSSTORM is extremely computationally expensive, which limits its practical application. Here, we develop a new algorithm (MempSTORM) based on two-dimensional spectrum analysis. With the same localization accuracy and recall rate, MempSTORM is 100 times faster than CSSTORM with â(1)-homotopy. In addition, MempSTORM can be implemented on a GPU for parallelism, which can further increase its computational speed and make it possible for online super-resolution reconstruction of high-density emitters.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Algoritmos
/
Processamento de Imagem Assistida por Computador
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article