Improving plant transformation using Agrobacterium tumefaciens.
Genet Mol Res
; 14(2): 6695-8, 2015 Jun 18.
Article
em En
| MEDLINE
| ID: mdl-26125878
ABSTRACT
Here, we report a quick and low-cost method to improve plant transformation using Agrobacterium tumefaciens. This method involves the use of physical wounding, ultrasound, and an increase in exposure time to the bacteria. We show how the transformation rate increased from 0 to 14% when an ultrasound pulse of 10 s was used in conjunction with 96 h of bacterial exposure in Eclipta alba explants.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Transformação Genética
/
DNA Bacteriano
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Plantas Geneticamente Modificadas
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Agrobacterium tumefaciens
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Caules de Planta
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Eclipta
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article