Effects of host response and antifungal therapy on serum and BAL levels of galactomannan and (1â3)-ß-D-glucan in experimental invasive pulmonary aspergillosis.
Med Mycol
; 53(6): 558-68, 2015 Aug.
Article
em En
| MEDLINE
| ID: mdl-26129890
ABSTRACT
Galactomannan and (1â3)-ß-D-glucan are useful biomarkers of invasive pulmonary aspergillosis (IPA). However, the effects of immunosuppression on levels of galactomannan or (1â3)-ß-D-glucan in IPA are not well understood or quantified. We therefore studied the simultaneous levels of galactomannan and (1â3)-ß-D-glucan in two rabbit models of experimental IPA (1) AraC-induced neutropenia in untreated (UC-AraC) and liposomal amphotericin B-treated (LAMB-AraC) rabbits; and (2) nonneutropenic cyclosporine-methylprednisolone immunosuppression in untreated (UC-CsA+M) and LAMB-treated (LAMB-CsA+M) rabbits. Simultaneous levels of galactomannan and (1â3)-ß-D-glucan were determined in bronchoalveolar lavage (BAL) fluid and serial serum specimens and correlated with pulmonary host response. Serum galactomannan index (GMI) and (1â3)-ß-D-glucan concentration-time-curves were higher in UC-AraC vs. UC-CsA+M (Mann-Whitney U-test, P < .05). Serum galactomannan and (1â3)-ß-D-glucan in treatment groups demonstrated therapeutic responses with similarly lower levels in comparison to UC (P < .01) in both models. Host differences did not affect BAL fluid GMI or (1â3)-ß-D-glucan but did affect galactomannan and (1â3)-ß-D-glucan levels in serum. The higher serum GMI and (1â3)-ß-D-glucan concentration-time-curves in UC-AraC correlated with extensive pulmonary infiltration by angioinvasive hyphae and minimal inflammation, while the lower concentration-time-curves in UC-CsA+M were associated with shorter and fewer hyphae in lung tissue and an intensive neutrophil response to Aspergillus hyphae. Thus, serum levels of galactomannan and (1â3)-ß-D-glucan in IPA depended upon immunosuppression, which also affected severity of infection and hyphal morphology, while BAL fluid galactomannan and (1â3)-ß-D-glucan were sensitive biomarkers not affected by host response.
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Base de dados:
MEDLINE
Assunto principal:
Líquido da Lavagem Broncoalveolar
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Beta-Glucanas
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Aspergilose Pulmonar Invasiva
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Mananas
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Antifúngicos
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article