Quantitative O-Glycomics by Microwave-Assisted ß-Elimination in the Presence of Pyrazolone Analogues.
Anal Chem
; 87(15): 7524-8, 2015 Aug 04.
Article
em En
| MEDLINE
| ID: mdl-26132640
ABSTRACT
O-Linked glycosylation of serine/threonine residues is a posttranslational modification of proteins and is essential for protein recognition and lipid functions on cell surfaces and within cells. The characterization of differently structured O-linked glycans (O-glycans) is particularly challenging because there is no known endoglycosidase for such groups. Therefore, chemical digestion approaches have been widely used; however, it is sometimes difficult to suppress unwanted side reactions. Recently, we reported a novel O-glycomics procedure using ß-elimination in the presence of pyrazolone analogues (BEP). In the present study, we describe a microwave (MW)-assisted BEP procedure for rapid and quantitative O-glycomic analysis. Following optimization of the reaction conditions, the MW-assisted BEP reaction substantially improved the recovery of total O-glycans from model glycoproteins (PSM) and the reaction time was reduced from 16 to 2 h. Combined with sequential solid-phase extractions, this MW-assisted BEP procedure enabled O-glycomic analyses of various biological samples.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
/
Pirazolonas
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Glicômica
/
Micro-Ondas
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article