PRIGo: a new multi-axis goniometer for macromolecular crystallography.
J Synchrotron Radiat
; 22(4): 895-900, 2015 Jul.
Article
em En
| MEDLINE
| ID: mdl-26134792
ABSTRACT
The Parallel Robotics Inspired Goniometer (PRIGo) is a novel compact and high-precision goniometer providing an alternative to (mini-)kappa, traditional three-circle goniometers and Eulerian cradles used for sample reorientation in macromolecular crystallography. Based on a combination of serial and parallel kinematics, PRIGo emulates an arc. It is mounted on an air-bearing stage for rotation around ω and consists of four linear positioners working synchronously to achieve x,â
y,â
z translations and χ rotation (0-90°), followed by a Ï stage (0-360°) for rotation around the sample holder axis. Owing to the use of piezo linear positioners and active correction, PRIGo features spheres of confusion of <1â
µm, <7â
µm and <10â
µm for ω, χ and Ï, respectively, and is therefore very well suited for micro-crystallography. PRIGo enables optimal strategies for both native and experimental phasing crystallographic data collection. Herein, PRIGo hardware and software, its calibration, as well as applications in macromolecular crystallography are described.
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2015
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Article