IMAC capture of recombinant protein from unclarified mammalian cell feed streams.

Kinna, Alexander; Tolner, Berend; Rota, Enrique Miranda; Titchener-Hooker, Nigel; Nesbeth, Darren; Chester, Kerry

Kinna, Alexander; Tolner, Berend; Rota, Enrique Miranda; Titchener-Hooker, Nigel; Nesbeth, Darren; Chester, Kerry.

Afiliação

Kinna A; Department of Oncology, University College London, UCL Cancer Institute, 72 Huntley Street, London, WC1E 6BT, UK.
Tolner B; Department of Biochemical Engineering, University College London, Gower Street, London, WC1E 6BT, UK.
Rota EM; Department of Oncology, University College London, UCL Cancer Institute, 72 Huntley Street, London, WC1E 6BT, UK.
Titchener-Hooker N; Department of Oncology, University College London, UCL Cancer Institute, 72 Huntley Street, London, WC1E 6BT, UK.
Nesbeth D; Department of Biochemical Engineering, University College London, Gower Street, London, WC1E 6BT, UK.
Chester K; Department of Biochemical Engineering, University College London, Gower Street, London, WC1E 6BT, UK.

Biotechnol Bioeng ; 113(1): 130-40, 2016 Jan.

Article em En | MEDLINE | ID: mdl-26174988

Assuntos

Cromatografia de Afinidade/métodos; Proteínas Recombinantes de Fusão/isolamento & purificação; Animais; Células CHO/metabolismo; Antígeno Carcinoembrionário/isolamento & purificação; Antígeno Carcinoembrionário/metabolismo; Sobrevivência Celular; Cricetulus; Microesferas; Proteínas Recombinantes de Fusão/metabolismo; Cloreto de Sódio/metabolismo

Palavras-chave

Chinese hamster ovary cells; IMAC; downstream processing; integrated processing; radial flow chromatography; recombinant protein

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas Recombinantes de Fusão / Cromatografia de Afinidade Limite: Animals Idioma: En Ano de publicação: 2016 Tipo de documento: Article

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